The complete mitochondrial genome of the poisonous mushroom Trichoderma cornu-damae (Hypocreaceae)

Abstract Trichoderma cornu-damae is a poisonous mushroom that contains trichothecene mycotoxins. The complete mitochondrial genome of this mushroom was determined using next-generation sequencing. This mitogenome is a circular molecule 94,608 bp in length with a GC content of 27.94% and contains 15 protein-coding genes, two rRNA genes (rnl and rns), and 25 tRNA genes. Phylogenetic analysis placed T. cornu-damae in the family Hypocreaceae group, which includes the genus Trichoderma. The mitogenome of T. cornu-damae will contribute to our understanding of the phylogeny, taxonomy, and population genetics of this mushroom.

Trichoderma cornu-damae (Pat.) Z.X. Zhu & W.Y. Zhuang 2014, a poisonous mushroom belonging to the Hypocreaceae family, is found in Korea, Japan, China, and Java, and the fruiting body is similar to that of a red pencil or deer horn (Gonmori et al. 2011;Zhu and Zhuang 2014;Kim et al. 2016). The major toxins of T. cornu-damae were identified as trichothecene macrolides, especially satratoxin H, and its 12 0 -acetate, 13 0 -acetate, and 12 0 ,13 0 -diacetate (Saikawa et al. 2001). In 1999, five people drank alcoholic drinks prepared with approximately 1 g of this poisonous mushroom and died two days later despite intensive treatment (Saikawa et al. 2001). In Korea, one person who ate this mushroom developed nausea, vomiting, and fever within three hours, showed hemorrhagic and necrotic changes in the lungs, and died within 12 days due to multiorgan failure (Jang et al. 2013). This mushroom is difficult to distinguish from the young fruiting bodies of the edible mushrooms Ganoderma lucidum and Cordyceps militaris; therefore, poisoning cases have occurred in Korea and Japan (Kim et al. 2016;Choe et al. 2018). This is the first study to report the complete mitogenome sequence of the lethal poisonous mushroom T. cornu-damae and the first report to determine the phylogenetic position of this mushroom.
The strain of T. cornu-damae (voucher number: KA19-0412C) used in this study was collected from the Korea National Arboretum (Pocheon, South Korea; N37 45 0 11.84 00 , E127 09 0 54.85 00 ). This sample was classified using the morphological method as per Zhu and Zhuang (2014), and through phylogenetic analysis using the ITS region of the DNA sequence. DNA for mitogenome analysis was extracted from mycelia isolated from this fruit body. Fruit body specimens and mycelia were deposited and maintained at the Korea National Arboretum (https://kna.forest.go.kr/, Dr. Chang-Sun Kim, changsun84@korea.kr). Total genomic DNA was extracted from mycelia cultured in potato dextrose agar media for 30 days in a dark environment using a GenEX Plant Kit (GeneAll, Seoul, South Korea), following the manufacturer's instructions, and sequenced using an Illumina HiSeq platform. High-quality paired-end reads (>20 Phred) were obtained after trimming and assembling de novo using the CLC genome assembler (v. 4.21, CLC Inc., Aarhus, Denmark). From the initially assembled contigs, those with sequences derived from the mitochondrial genome were further processed to generate a single draft sequence, as previously reported by Lee et al. (2018). The draft sequence was manually corrected and gap filled by mapping a series of paired-end reads. The final complete mitogenome sequence was annotated using GeSeq (https://chlorobox.mpimp-golm. mpg.de/geseq-app.html) (Tillich et al. 2017) and manual curation was performed using the Artemis annotation tool (Rutherford et al. 2000) with NCBI BLASTN searches.
For phylogenetic analysis, the DNA sequences of 11 coding genes (atp6, atp8, atp9, cob, cox1, cox2, nad2, nad3, nad4, nad5, and nad6) in the mitogenome of T. cornu-damae and 15 other fungal species were used. The general time reversible (GTR) model and maximum-likelihood method of MEGA 7 with 1000 bootstrap replicates were used ). Phylogenetic analysis placed T. cornu-damae into the family Hypocreaceae, which includes the genus Trichoderma, in comparison to other species (Figure 1). The mitogenome of T. cornu-damae reported in this study will contribute to the understanding of its phylogeny, taxonomy, and population genetics.

Ethical approval
The study does not involve humans and animals. This study does not need ethical approval or permissions to collect the sample.

Data availability statement
The data that support the findings of this study are openly available in GenBank of NCBI at https://www.ncbi.nlm.nih.gov/ under the accession no. MW525445. The associated BioProject, SRA, and Bio-Sample numbers are PRJNA739541, SRR18250171, and SAMN19791815, respectively.